Biological comprehensive analysis combined instrument

Function Introduction

Functional introduction

hardware system

Biological comprehensive analysis combined instrumentAdopting a fully enclosed dark box to isolate the interference of ambient light, completely eliminating the light spot and halo phenomenon formed by the refraction of stray light in the glass culture dish, providing the necessary light and shadow conditions for colony counting.

Colony count

The adjustment function of colony display mode, including particle contour, particle number, and particle filling, can be set in color according to user needs.

One click statistics

10 mode one click counting

Rapid colony count:

Single color rapid statistics: Conduct rapid statistics on single color colonies, multi-color colonies, small colonies, large colonies, colored colonies, and spreading bacteria.

Multi color bacteria automatic stacking counting: By using a color pen to extract the color of the target colony, all colonies of the same color in the selected area can be automatically segmented. This algorithm is suitable for rapid statistics of colonies of specific colors.

Microbe statistics: adapted to the statistics of small bacterial colonies.

Mega bacteria statistics: rapid statistics of large bacterial colonies within a region, with a focus on large bacterial colony statistics.

Statistics of bacteria resembling the background color: When the bacterial colonies in the area are similar in color to the background color of the culture dish, statistics of bacteria resembling the background color are used.

Adhesive bacteria statistics: suitable for rapid statistics of colonies with a single background color, no color, and blurry edges.

Scattered bacteria statistics: Quickly count colonies with clear edges and scattered patterns.

Grid filter membrane: including 3M paper colony statistics, filter membrane paper colony statistics, and solid line paper colony statistics.

One click measurement of antibacterial zone

One click,Accurately identify the "boundary line" between the antibacterial transparent area and the underlying bacteria

Antibiotic potency determination

Provide one dose method, two dose method, and three dose method. The one dose method complies with the United States Pharmacopeia, while the two dose and three dose methods comply with the Chinese Pharmacopoeia 2015 edition.

Data Security and Management

1) Multi user login system, each account forms independent data, and the data is saved for a long time

2) The statistical results are output in PDF format, and the original data cannot be changed

3) Equipped with audit tracking function, the software automatically records every step of operation by the operator, facilitating the traceability of subsequent result data

4) Compatible with CFR 21 Part 11: System Security, Operational Control, File Management

Main performance indicators

Performance

1. Microscopic imaging device

10 million pixel ultra clear CMOS camera?

Data interface: USB 3.0

Microscope adapter

Olympus CX31 Trinocular Microscope

2. Host imaging device

16 million pixel ultra clear CMOS camera

5-megapixel high-definition lens

3. Software functions

① Colony analysis

1)One click classification statistics:

RIDA ™ One click counting

Compact Dry ™ One click counting

One click counting of bacteria with similar backgrounds

One click counting of microorganisms

One click counting of dispersed bacteria

One click counting of adhesive bacteria

One click counting of large bacterial colonies

2)Auxiliary statistical tools:

Manual correction: Click with the mouse to add or delete missing colonies

Intelligent correction: Based on one click statistics, intelligent correction can be performed

Measurement tools: angle, line segment, area, curve

Removal of contaminating bacteria (impurities): Remove impurities based on color, diameter, and roundness

Single colony morphology analysis: Clicking on a single colony can reveal information such as roundness, diameter, circumference, and area of the colony

All colony morphology: After statistics, the roundness, diameter, circumference, area, and other information of all colonies on the plate can be obtained

Colony size classification: After counting, display the colony size in 25 categories based on the contour size of each colony

Conversion of total number of bacterial colonies in the sample: Based on the actual diameter of the culture dish and the dilution of the sample, automatic conversion is achieved

② Antibacterial zone and potency measurement

1)Classification one click measurement:

Automatic measurement: suitable for edge detection with clear edges and standard circular antibacterial circle contours

Quasi circle filling: Determine the center of the circle based on the arc and simulate the entire circle

Manual measurement: Click on three points on the circular arc of the antibacterial zone with the mouse to form a circle, suitable for antibacterial zones with blurred edges

2)Antibiotic potency determination

One dose method potency testing: suitable for the United States Pharmacopeia

Two dose method potency testing: suitable for the Chinese Pharmacopoeia 2010 and 2015 editions

Triple dose method potency testing: suitable for Chinese Pharmacopoeia 2010 and 2015 editions

3)Instrument detection accuracy

Repetitive self check: relative error ≤ 0.01%, relative error of repeated measurements ≤ 0.001mm

Uniformity self check: relative error ≤ 0.03%

Measurement difference between stations ≤ 0.1%

Relative error of repeated measurement of potency ≤ 0.2%

Relative error of potency measurement ≤ 0.2%

③ Microscopic Cell Analysis

1)image processing

By performing resolution enhancement processing on the original image that matches its features, the image becomes clearer and the edges are more prominent, facilitating the observation and recognition of subtle structures in the image

Image brightness, contrast, saturation, RGB color can be adjusted arbitrarily, and grayscale and negative phase diagrams can be converted

Compensate for the distorted parts of the image through various mathematical methods such as linear compensation, logarithmic compensation, Bell compensation, etc., to make the image clearer

Six filtering methods, including Gaussian filtering, low-pass filtering, and median filtering, effectively improve image clarity

Nonlinear mathematical morphology processing such as corrosion, expansion, opening, and closing

2)measure

Capable of online calibration of the system to achieve measurement

Online measurement of particle diameter, length, curvature, angle, arbitrary curve, area, etc

3)Cell count

Automatic particle counting and display morphological parameters such as area, perimeter, diameter, roundness, etc. of each particle

You can choose any shape area such as rectangle, circle, umbrella for statistics

Set the diameter range and count particles of a specific size

Filter specific particles based on chromaticity, brightness, and saturation

Click with the mouse to add or delete particles, convenient and fast

Can batch count multiple microscopic images

④ Data Security and Reporting

Multi user login system, each account forms independent data, and the data is saved for a long time

The statistical results are output in PDF format, and the original data cannot be changed

Equipped with audit tracking function, the software automatically records every step of operation by the operator, facilitating the traceability of subsequent result data

Automatically save each batch of photos, statistical identification, and statistical data

configuration

Configuration

Camera conversion interface for 10 megapixel color microscope CMOS camera and three lens microscope

16 million high-definition CMOS camera

MAS-H2 colony counting host

Zstream colony counting analysis system, inhibition zone determination and potency analysis system, microscopic image analysis system

Brand business all-in-one computer

Olympus CX31 three eye microscope (optional with other three eye microscopes)